cxcl10  (R&D Systems)

Journal: Journal of Advanced Research

Article Title: The pro-cancer and immunosuppressive activity of macrophage-transformed cancer-associated fibroblasts in oral squamous cell carcinoma

doi: 10.1016/j.jare.2025.07.027

Figure Lengend Snippet: MMTs recruit a large number of MDSCs through CXCL10 pathway. A Significantly upregulated genes in MMTs versus non-MMT fibroblasts from OSCC database (GSA-Human: HRA007439). B Differences in CXCL10 expression between non-MMT fibroblasts and MMTs were demonstrated in HNSCC (figure S1). C CXCL10 expression and release were detected by PCR and ELISA. D-H Exogenous CXCL10 was added to BMDMs, while the CXCL10 inhibitor AMG487 was administered to MMTs, followed by co-culturing with MDSCs. The migration of MDSCs was evaluated using transwell chambers and crystal violet staining ( D ). Flow cytometry was used to detect cell apoptosis ( E ), macrophage markers (F4/80 + CD11b + ) ( F ), as well as CFSE for assessing T cell proliferation inhibition ( G ). PCR and ELISA were employed to measure the expression of immunosuppressive factors IL10 and iNOS ( H ). I, J Tumor tissue photographs ( I ) and the tumor growth curve were taken and subjected to statistical analysis regarding volume and weight ( J ). K-M Flow cytometry was used to detect the proportion of MDSCs in the spleen ( K ) and tumors ( L ) of each group, while immunofluorescence staining ( M ) revealed the densest field of MDSCs within the tumor tissues across all groups. Data are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs experimental group. “ns” represents no statistical significance. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: According to the manufacturer's instructions, the cytokine concentrations of TGFβ1, iNOS, IL-10, and CXCL10 in the supernatant were determined using ELISA kits (Boster, California, USA).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Migration, Staining, Flow Cytometry, Inhibition, Immunofluorescence

Journal: bioRxiv

Article Title: Immune cells adapt to distinct stem cell niches to govern tissue homeostasis

doi: 10.64898/2026.01.28.701831

Figure Lengend Snippet: a , GeneOntlogy (GO) terms up- and down-regulated in uHF-SC upon in vivo intradermal administration of LTBR-agonist compared to isotype control injected mice. b , Normalized quantification of number of CD4 + and CD8 + T cells migrated through a transwell membrane towards the supernatant collected from uHF-SC treated with LTBR agonist or isotype control. Each dot represents one technical replicate from four independent experiments. c , Normalized quantification of number of CD8 + T cells migrated through a transwell membrane towards the supernatant collected from Bulge-SC, IFE-SC or uHF-SC treated with LTBR agonist or isotype control. Each dot represents one technical replicate from three independent experiments. d , Left: Representative max projections of whole mount immunofluorescence of the bulge, uHF and IFE compartment of wild type mice showing distribution of CD4 + cells (in white). Bulge cells are marked by Krt24 in green and upper hair follicle cells are marked by LRIG1 in light blue. Right: Quantification of the average distance of CD4 + and CD8 + T cells from Krt24 and LRIG1 quantified upon surface rendering, n =2 mice. e , qPCR analysis of Cxcl10 and Cxcl16 expression Bulge-SC, uHF-SC and IFE-SC FACS-sorted from wild type mice. Each dot represents one mouse from two independent experiments. f , Representative images of MSA plates showing bacterial colonies grown after swabbing the back skin of Ltb-CreER x R26-DTA and R26-DTA (ctrl) mice associated with S. epidermidis . Data in b and d are analysed by unpaired two-tailed Student’s t- test. Data in c and e are analysed by ordinary one-way ANOVA with Tukey’s multiple comparison post-test. p -values are indicated in each figure and data are represented as mean with SEM.

Article Snippet: When indicated, 50μg/ml of InVivoMab anti-mouse CXCL10 (IP-10, BioXcell) was added to the lower chamber.

Techniques: In Vivo, Control, Injection, Membrane, Immunofluorescence, Expressing, Two Tailed Test, Comparison